Orion joined the Adamala lab in early 2019; since then, they have focused on the development of tools for synthetic cell engineering, rational RNA design, and using those synthetic biology techniques to elucidate viroid and plastid evolution. Orion joined the BMBB department as a PhD student in 2022.
Judee received her B.S. in Microbial Biology from the University of California, Berkeley in 2014. Although she did do research in her undergraduate years, she didn’t find her true interest in environmental microbiology and its applications until she began researching at the U.S. Department of Agriculture and start-up companies like Pivot Bio and Wild Earth. In 2018, she made the move to the University of Minnesota’s graduate program in Biochemistry, Molecular Biology, and Biophysics where she joined the Adamala-Engelhart Lab. She is interested in furthering the functionality of synthetic cells for use in environmental biotechnology applications. Likewise, she would like to use synthetic cell mechanisms to consider (or reconsider) the origins of life on Earth or potentially in extraterrestrial systems. When she’s not in lab, she likes to read, watch The Office, backpack, and drink beer.
Contact: sharo112@umn.edu
Wakana received her B.S. in Agriculture at Hokkaido University, Japan in 2018. She began her research career as an undergraduate under Dr.Takasuka, studying biomass-degrading enzymes, and Dr. Kato, studying the discovery of bioactive natural compounds.
In 2018, Wakana started graduate school at the University of Minnesota, and joined Dr. Kate Adamala’s lab. She is interested in enzyme engineering for natural product synthesis and gene-based tool development for biocomputer.
Contact: sato0055@umn.edu
The HTGAA course is based on the classic Fab-Lab “Hot to Make Almost Anything” fabrication class.
BioAcademy is the best resource to learn basisc of bioengineering, including specific applications of most cutting edge techniques. We run a synthetic cell module, including overwiew of the field of synthetic life and examples of synthetic cell based tools for biological engineering.
Excellent resource for beginning and advanced DIY biological engineers, biospaces and self-taught biohackers, as well as interesting supplement for anyone studying basics of modern biological engineering.
Build-a-Cell is an international community of scientists and policymakers working on building synthetic cells. We facilitate collaborations between groups in different areas of synthetic cell research, we engage with scientists in other disciplines to promote use of synthetic cell tools, and we provide information for the general public.
Our lab participated in every one of the bi-annual Build-a-Cell workshops, and we are actively pursuing collaborations with many Build-a-Cell members.
Kate Adamala is the co-founder and leader of Build-a-Cell.
STAT magazine published nice overview of efforts to build artificial life, From chemicals to life: Scientists try to build cells from scratch.
Details, including list of member labs, working groups, workshops and all resources can be found at www.buildacell.org.
The HTGAA course is based on the classic Fab-Lab “Hot to Make Almost Anything” fabrication class.
BioAcademy is the best resource to learn basisc of bioengineering, including specific applications of most cutting edge techniques. We run a synthetic cell module, including overwiew of the field of synthetic life and examples of synthetic cell based tools for biological engineering.
Excellent resource for beginning and advanced DIY biological engineers, biospaces and self-taught biohackers, as well as interesting supplement for anyone studying basics of modern biological engineering.
Astrobiology is studying past, present and future of the life on the Universe. Synthetic cells are the perfect tool to answer many of the questions typically asked by astrobiologists, including engineering synthetic cells to resemble earliest terrestrial life forms for studying of the origins of life on Earth, engineering synthetic cell systems to explore possibilities of biochemistries yielding life on other planets, and exploring possible future evolution scenarios for various life forms. Using synthetic cells we can ask many questions about “weird life” events and alternative life forms, questions that would be nearly impossible to answer studying modern, complex terrestrial cells.
Our work is driven by the question of what is the multidimensional niche space for life, and what are the molecular mechanisms of these boundary physicochemical conditions of life? This leads naturally to the questions of how does terrestrial life responds to changes at those boundary conditions, and can synthetic biology be used to expand these? We aim at defining comprehensive matrix describing mechanisms in which environment restricts basic processes of biology, and developing framework for studying and designing living systems around those limitations.
Understanding what separates life from non-life is critical to solving some of the great outstanding questions in science, such as how life first emerged and how we might unambiguously detect life on other worlds. The transition from non-living to living matter has been notoriously difficult to quantify, limiting our ability to develop the necessary theory for understanding life or its universal properties. One of the most significant challenges is the complexity of the examples of life available to study, which represent the product of more than 3.5 billion years of evolution. Systems representing the intermediate stages of complexity between simple chemistry and modern biology are only now becoming accessible to study in the lab with advances in systems chemistry and minimal synthetic life, respectively. These have not yet been connected to explore the uncharacterized landscape between simple chemical systems and the much more complex biochemical architectures characteristic of life today. Our project is an unprecedented opportunity to systematically study the full pathway from non-life to life through a synthesis of bottom-up chemistry, top-down minimal biology and fundamental theory. We aim to build very simple systems from chemical and biological parts, and by studying varying stages of programmed complexity and autonomy, systematically evaluate the transitions in information through which these become more life-like, self-referential entities.
Thank you for support: Templeton Foundation.
We are developing solutions for automating and scaling thropought of synthetic cell epxeriments. Microfluidic rigs help us work faster, iterating more genetic circuit designs with automated liposome formation and streamlined detection of protein expression.
Our first rig recently started producing droplets with fluorescent RNA.
Most of the efforts of biological research, both in studying healthy organisms as well as disease states, is focused either on studying the whole live cell (or organism), or on studying isolated reactions between few purified and well defined components in vitro. Most biological processes are not isolated events of interaction between few components, but rather complex interconnected networks built of often multifunctional nodes (proteins acting on many targets). The in vitro studies give results that are less relevant to natural biology – since an experiment with a few purified components does not acknowledge the vast complexity of a natural system. On the other hand, live cell studies are notoriously hard to reproduce and interpret, due to the variability between live subjects, as well as due to the inherent complexity of biology (cross-talk between the studied process and other pathways, or background signal from unrelated processes is often present).
Synthetic minimal cells deliver a solution bridging the existing gap between in vitro and live cell research: use synthetic minimal cells to investigate multicomponent gene pathways, combining the advantages of in vitro systems with the relevancy and complexity approaching that of whole cell studies.
Reading and controlling cells is the core purpose of modern synthetic biology, and the overarching goal of all biomedical studies. Both studying mechanisms of most diseases, as well as investigating healthy cellular processes, is currently done as either in vitro or live cell experiments. In vitro research methods are easy to use, cheap and efficient way to obtain information about behavior of specific, well defined protein or nucleic acid complexes or single enzymes, or to characterize small molecule interactions between metabolites or drugs and their specific biological targets. However, since life is structured in complexes that involve many components organized in precise 3D assemblies, the in vitro experiments often only deliver information about small snapshot of this complex, natural system. Studies of live cells allow to obtain truly biologically relevant information about complex pathways, but at significantly higher cost, and with results that are harder to interpret and often less reproducible. The variability between live cell subjects and the underlying intricacy of interconnected biological networks constantly interacting with each other makes signal measured in live cell experiments often more noisy and the experiment itself difficult to design.
Synthetic minimal cells offer a platform that allows studying complex genetic pathways, while keeping the complexity of the system at a level that still allows us understanding fully what the system contains and how to engineer it. Our research focuses on building tools for general use in many areas of synthetic biology, as well as studying some specific cases of complex biological processes, both healthy and diseased, that are not accessible by studying natural complex cells.
