Category: publ

A gene expression control technology for cell-free systems and synthetic cells via targeted gene silencing and transfection;
Wakana Sato, Melanie Rasmussen, Nathaniel Gaut, Mahima Devarajan, Kaitlin Stokes, Christopher Deich, Aaron E. Engelhart, Katarzyna P. Adamala
Biotechnology and Bioengineering, 2023, 1– 12.
http://doi.org/10.1002/bit.28422

New Aequorea fluorescent proteins for cell‐free bioengineering;
Christopher Deich, Nathaniel J. Gaut, Wakana Sato, Aaron E. Engelhart, Katarzyna P. Adamala
ACS Synth. Biol. 2023,
https://doi.org/10.1021/acssynbio.3c00057

Parasites, infections and inoculation in synthetic minimal cells;
Brock Cash, Nathaniel J. Gaut, Christopher Deich, Laura L. Johnson, Aaron E. Engelhart, Katarzyna P. Adamala
ACS Omega 2023, https://pubs.acs.org/doi/10.1021/acsomega.2c07911

T7Max transcription system
Christopher Deich, Brock Cash, Wakana Sato, Judee Sharon, Lauren Aufdembrink, Nathaniel J. Gaut, Joseph Heili, Kaitlin Stokes, Aaron E. Engelhart, Katarzyna P. Adamala
J Biol Eng 17, 4 (2023)
doi.org/10.1186/s13036-023-00323-1

A ubiquitous amino acid source for prokaryotic and eukaryotic cell-free transcription-translation systems
Nagappa LK, Sato W, Alam F, Makrydaki E, Chengan K, Smales M, von der Haar T, Polizzi KM, Adamala KP, and Moore SJ
Front. Bioeng. Biotechnol. 2022, 
https://doi.org/10.3389/fbioe.2022.992708

Engineering Ribosomes to Alleviate Abiotic Stress in Plants;
Leticia Dias-Fields and Katarzyna P. Adamala
Plants 2022, 11(16), 2097
https://doi.org/10.3390/plants11162097

Expanding luciferase reporter systems for cell-free protein expression;
Wakana Sato, Melanie Rasmussen, Christopher Deich, Aaron E. Engelhart, Katarzyna P. Adamala
Sci Rep 12, 11489 (2022), https://doi.org/10.1038/s41598-022-15624-6

Traditional protocols and optimization methods lead to absent expression in mycoplasma cell-free gene expression platform
Andrei Sakai, Christopher R. Deich, Frank H. T. Nelissen, Aafke J. Jonker, Daniela M. de C. Bittencourt, Christopher P. Kempes, Kim S. Wise, Hans A. Heus, Wilhelm T. S. Huck, Katarzyna P. Adamala, John I. Glass
Synthetic Biology, ysac008 , https://doi.org/10.1093/synbio/ysac008

Akaby – cell-free protein expression system for linear templates
Wakana Sato, Judee Sharon, Christopher Deich, Nathaniel Gaut, Brock Cash, Aaron E Engelhart, Katarzyna P Adamala
PLoS ONE 17(4): e0266272. https://doi.org/10.1371/journal.pone.0266272

Diffusion control in biochemical specificity;
Jose L. Alejo, Christopher P. Kempes, Katarzyna P. Adamala
Biophysical Journal (2022), Volume 121, Issue 8, 19 April 2022, Pages 1541-1548
https://doi.org/10.1016/j.bpj.2022.03.005